
Isolation of bacteriophages with lytic activity against a newly identified Pantoea agglomerans
Author(s) -
Nataliia Korniienko,
E. A. Dukhno,
А. В. Харіна,
I. G. Budzanivska
Publication year - 2019
Publication title -
vestnik kievskogo nacionalʹnogo universiteta imeni tarasa ševčenko/vìsnik
Language(s) - English
Resource type - Journals
eISSN - 2308-8036
pISSN - 1728-3817
DOI - 10.17721/1728_2748.2019.77.50-55
Subject(s) - pantoea agglomerans , bacteria , bacteriophage , biology , microbiology and biotechnology , lytic cycle , myoviridae , pathogenic bacteria , pantoea , antibiotics , isolation (microbiology) , human pathogen , virus , escherichia coli , virology , 16s ribosomal rna , genetics , gene
In a consequence of agricultural human activity, a set of phytopathogenic bacteria gain new properties and ability to cause diseases in animal and human organisms. Moreover, bacterial loss of sensitivity to antibiotics becomes more increasing threat. The most effective alternative method of processing of plants are bacteriophages. The aim of this work is isolation and identification of a vegetable enterobacteria and search of its specific bacteriophages. Methods: biochemical identification of bacteria, analysis on sensitivity to antibiotics by means of disks, titration and accumulation of virus, electronic microscopy. Results: from onions samples with symptoms of a bacteriosis several bacteria were isolated. One of them was identified as Pantoea agglomerans. The sensitivity of this isolate to antibiotics was investigated, the resistance to cefalexin and norfloxacin is revealed. The bacteriophage specific to this bacteria is isolated from waste waters. The morphology of a bacteriophage is investigated by means of electronic microscopy, the virus belongs to the Myoviridae family. Phytopathogenic properties of bacteria and the antibacterial activity of phage isolate were investigated on potatoes in vitro. P. agglomerans led to development of a bacteriosis on potatoes cubes, and the isolated bacteriophage successfully inhibited its growth. Conclusions: This study demonstrated that common vegetables such as onions could be a source of human pathogenic bacteria. In this work, we isolated P.agglomerans, member of family Enterobacteriaceae. Taking into account that this bacteria was unsensitive to some antibiotics, it can be regarded as an alarming sign. The use of bacteriophages could solve problems of antimicriobial resistance and protecting of crops from bacterial infections. Isolated bacteriophage from waste waters inhibited growth of P.agglomerans in vitro showing that it could be considered as a part of phage drugs.