Open AccessCALLUS INDUCTION IN VITRO OF CACTACEAE FAMILY
Author(s) -
Valentyna Maliarenko,
А. В. Голубенко
Publication year - 2017
Publication title -
vìsnik - kiïvsʹkij nacìonalʹnij unìversitet ìmenì tarasa ševčenka. bìologìâ
Language(s) - English
Resource type - Journals
ISSN - 1728-2748
DOI - 10.17721/1728_2748.2017.73.54-56
Subject(s) - callus , sucrose , explant culture , ascorbic acid , murashige and skoog medium , botany , chemistry , acetic acid , in vitro , biology , horticulture , food science , biochemistry
Our research goal has been to find the optimal nutrient media for initiation of the primary callus in the species of the Cactaceae family. Common methods of plant biotechnology were used. Primary explants of the cacti were cultivated on Murashige and Skoog medium (MS medium). The content of macro- and microelements has been diluted twice (½ MS) and the vitamins (B1 and B6 – 0.5 mg/l, PP – 1 mg/l) were added, as well as 100 mg/l meso-isonitol and 20 g/l of sucrose. It was determined that callus formation formed efficiently when cultivated on half MS media with 20 g/l sucrose, 3 mg/l 6- benzylaminopurine, 0,2 mg/l indole-3-acetic acid,0,1 mg/l α-napthaleneacetic acid and 5 mg/l ascorbic acid. It was discovered, that for initiation of tissue differentiation and cacti callus formation, high concentrations of cytokinine-active growth regulators are required.