Open AccessMolecular genetic changes in kidney tissue in patients with COVID-19
Author(s) -
Г А Демяшкин,
Artemii Mingazov,
Е. А. Каприна,
Vladimir Shchekin,
П. В. Шегай
Publication year - 2021
Publication title -
issledovaniâ i praktika v medicine
Language(s) - English
Resource type - Journals
eISSN - 2410-1893
pISSN - 2409-2231
DOI - 10.17709/2410-1893-2021-8-3-4
Subject(s) - furin , kidney , covid-19 , biology , virology , real time polymerase chain reaction , polymerase chain reaction , messenger rna , gene , gene expression , viral load , microbiology and biotechnology , virus , immunology , medicine , endocrinology , genetics , enzyme , biochemistry , disease , infectious disease (medical specialty)
Purpose of the study . Assessment of the molecular genetic profile of cytological processes in the kidney tissue of patients with COVID‑19. Material and methods . Kidney fragments from patients with confirmed COVID‑19 (n = 96) were studied by real-time polymerase chain reaction to determine the expression of SARS-CoV‑2 viral RNA and genes encoding protein complexes: ACE‑2 and Furin. Results . In patients affected by COVID‑19, the presence of coronavirus genetic material in kidney tissue was recorded, as well as increased expression of ACE‑2 (7.49 ± 0.27, p < 0.01) and Furin 2.0 times (8.59 ± 0.65, p < 0.01,) compared with the control group (3.9 ± 0.48, p < 0.01 and 4.2 ± 0.8, p < 0.01, respectively), which creates favorable conditions for the invasion of SARS-CoV‑2. Conclusion . According to the results of RT-PCR for SARS-CoV‑2, assessment of the expression of ACE‑2 and Furin, it is possible with a high degree of probability to assert about the viral load and the vulnerability of the kidneys, since these proteins are informative markers of viral damage. Elderly people with increased vulnerability to SARS-CoV‑2.