Open AccessChanges in human sperm motility characteristics in the presence of protein-peptide complex
Author(s) -
В. В. Евдокимов,
E. A. Efremov,
P. A. Elistratov,
I. A. Yamskov,
A. F. Askerov,
V. P. Yamskova,
V.B. Turovetsky
Publication year - 2018
Publication title -
andrologiâ i genitalʹnaâ hirurgiâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.138
H-Index - 1
eISSN - 2412-8902
pISSN - 2070-9781
DOI - 10.17650/2070-9781-2018-19-3-70-74
Subject(s) - motility , sperm motility , peptide , incubation , sperm , andrology , biology , semen , chemistry , biochemistry , anatomy , microbiology and biotechnology , medicine
The study objective is to examine the changes in motility of human spermatozoa under the effect of a protein-peptide complex extracted from cattle testes (Bos taurus). Materials and methods . The protein-peptide complex was extracted from testes tissue obtained during butchering of cattle at meat processing plants of the Moscow Region. Human spermatozoa were obtained from the ejaculate in the usual way. From the ejaculate sample, 1 ml was collected for the experiment and control. The protein-peptide complex was added to a test tube in concentrations of 10–8, 10–9, and 10–12 mg/ml. For every concentration, 10–12 experiments were performed. General and active motility were evaluated after 30 min, 1 and 3 hours of incubation at room temperature. Results and conclusion . The experiments have shown that in the presence of the protein-peptide complex, spermatozoa motility increased by 10–30 % relative to the initial value at 30 minutes, and this change persisted for 3 hours of observation. The change depended on the complex concentration: the largest increase was observed at 10–12 mg/ml. Higher complex concentrations did not have a positive effect on spermatozoa motility.