Open AccessIdentification of phenolic compounds of dry extract of dandelion herb and large burdock leaf tea
Author(s) -
Л. М. Федосеева,
Ю. И. Чистова
Publication year - 2019
Publication title -
rossijskij bioterapevtičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 1726-9792
pISSN - 1726-9784
DOI - 10.17650/1726-9784-2019-18-2-73-77
Subject(s) - dandelion , chemistry , umbelliferone , chlorogenic acid , caffeic acid , herb , chromatography , phenolic acid , ferulic acid , high performance liquid chromatography , coumaric acid , coumarin , organic chemistry , traditional medicine , medicinal herbs , antioxidant , medicine , alternative medicine , pathology , traditional chinese medicine
The purpose of this work is to study of phenolic compounds in the dry extract of dandelion herb and large burdock leaf tea. Materials and methods . The separation and identification of phenolic compounds of dry extract of dandelion herb and large burdock leaf tea by thin-layer chromatography and high-performance liquid chromatography with UV-detectionhas been carried out. Results . As a result of research, it has been established that during TLC the optimal system for the separation of phenolic compounds is the ethyl acetate – formic acid – water system (10:2:3). On the chromatogram four spots were found corresponding to the value of Rf and fluorescence in UV-light to flavonoids of the flavone group and phenolic acids (chlorogenic and caffeic acids). For further identification of phenolic compounds using HPLC, eight peaks were found, which in terms of retention time and spectral characteristics correspond to phenologlycosides, chlorogenic acid, caffeic acid derivatives, ferulic acid, umbelliferone. Conclusions . Thus, the dry extract of dandelion herb and large burdock leaf tea contains hydroxycinnamic acids and their derivatives, compounds of coumarin nature, phenologlycosides.