Open AccessLymphocytes blast-transformation reaction: modification for allergological practice
Author(s) -
Манина Ирина Владимировна,
В Ю Сергеев,
Н В Голубцова,
А. И. Сергеев
Publication year - 2018
Publication title -
rossijskij bioterapevtičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 1726-9792
pISSN - 1726-9784
DOI - 10.17650/1726-9784-2018-17-2-88-92
Subject(s) - pentium , software , computer science , identification (biology) , automation , digitization , transformation (genetics) , embedded system , operating system , immunology , medicine , engineering , computer vision , biology , mechanical engineering , biochemistry , botany , gene
. Lymphocytes blast-transformation reaction (RBLT) is necessary for patient’s inspection with immunologic infringements. It is applied in the different fields of medicine to identification of a sensitization to antigens (allergens). Research objective – to modify and automate RBTL for application in routine laboratory practice. Materials and methods. RBTL, antigens (allergens). Software: Windows 7; Intel Pentium G4500 CPU. Digital CMOS video camera, Mikmed-6 microscope. Results. We analyzed difficulties when using RBTL in laboratory allergological practice. We created program files for automation of RBTL. Conclusion. We adapted and automated RBTL for laboratory diagnostics using.