Open AccessDNA-markers for genetic monitoring of laboratory mouse
Author(s) -
Yu. M. Bukreev,
Е. Н. Кособокова,
S. S. Kardashova,
M. V. Pinyugina,
В. С. Косоруков
Publication year - 2017
Publication title -
rossijskij bioterapevtičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 1726-9792
pISSN - 1726-9784
DOI - 10.17650/1726-9784-2017-16-3-86-91
Subject(s) - genetic monitoring , microsatellite , allele , biology , genetics , computational biology , microbiology and biotechnology , gene
. The use of standardized model is required at preclinical researches of drug safety and efficiency. Apart of veterinary control the leading laboratories of the world use genetic monitoring of animals. Objective: to develop the method for detecting genetic contamination of laboratory mouse based on microsatellite DNA markers analysis. Materials and methods. We tested two lines of mice C57BL/6J and BALB/cJLac. We used 5 primers: (GAG)6C, (CTC)6C, (CTC)6A, AG9-1, AG9-2. We weighted the results of рolymerase chain reaction using 1.5 % agarous gel. Results. Three of tested primers allowed to distinguish 2 lines of mice statistically reliably. Two mice lines were bred to control the results. Our data shows track of every line gives a boots in assortment of alleles in locuses. We composed a table of alleles assortment in locuses for every line based of results. Conclusion. Our results prove that this method provides fast, reliable and cost-effective way of genetic monitoring.