Open AccessANALYTICAL CHARACTERISTICS OF FLUORESCENT SUSPENSION NANOCRYSTAL-ENCODED MICROARRAY ADAPTED FOR SIMULTANEOUS QUANTITATIVE DETECTION OF FREE AND TOTAL PSA IN SERUM SAMPLES
Author(s) -
Kristina Brazhnik,
З. А. Соколова,
М. А. Барышникова,
Regina Bilan,
Igor Nabiev,
A V Sukhanova
Publication year - 2015
Publication title -
rossijskij bioterapevtičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 1726-9792
pISSN - 1726-9784
DOI - 10.17650/1726-9784-2015-14-4-31-38
Subject(s) - detection limit , reproducibility , multiplexing , suspension (topology) , dna microarray , microarray , computational biology , biological system , computer science , chemistry , chromatography , biology , mathematics , homotopy , pure mathematics , telecommunications , biochemistry , gene expression , gene
Multiplexed suspension systems of anew generation are capable to provide precise quantitative profiling of mul- tipledisease-specific markers in human body fluids. We have developed suspension microarrays based on microbeads encoded with fluorescent nanociystals, that show undeniable advantages overavailable analogues in terms of improved multiplexing capabilities, physical and optical properties, low cost and simplicity of analysisperformance. We have adapted QD-encoded suspension microarrays for the simultaneous detection of two forms of prostate-specific antigen (PSA) in human serum by means of classical flow cytometiy. In the present study, we describe in detail the designed system properties including evaluation the microarray for quantitative analysis of serum markers in comparison to standard clinical approach ELISA, as well asestimation of the most important analytical characteristics, such as analytic sensitivity (limit of detection), reproducibility, reliability and accuracy of the analysis, the linear rangesfor detectable- marker concentrations. Experimental data suggested that the developed diagnostic system quantifies two forms of PSA in blood serum samples of patients with high accuracy, precision and reliability.