Open AccessLYMPHOCYTE SUBSETS IN PERIPHERAL BLOOD OF HER2-POSITIVE AND TRIPLE NEGATIVE BREAST CANCER PATIENTS
Author(s) -
А. И. Черткова,
Е. Г. Славина,
Л. Г. Жукова,
Inna Ganshina,
M. F. Okruzhnova,
Э. К. Шоуа,
В А Нуртдинова,
А А Борунова,
З. Г. Кадагидзе
Publication year - 2015
Publication title -
rossijskij bioterapevtičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 1726-9792
pISSN - 1726-9784
DOI - 10.17650/1726-9784-2015-14-2-47-52
Subject(s) - cytotoxic t cell , immunophenotyping , flow cytometry , monoclonal antibody , immunology , breast cancer , population , foxp3 , lymphocyte , medicine , t lymphocyte , triple negative breast cancer , antibody , antigen , cancer research , cancer , immune system , biology , in vitro , biochemistry , environmental health
The aim of this study was to investigate the relationship between the original amount of basic lymphocyte subpopulations in peripheral blood and the results of therapy in patients with HER2+ and triple negative breast cancer. Before treatment was conducted immunophenotyping of peripheral blood lymphocytes by flow cytometry using a panel of monoclonal antibodies to surface markers and intracellular lymphocyte antigen FOXP3 and determining the cytotoxic activity of NK-cells. Cytotoxic activity was determined with MTT colorimetric test against K-562 cells. Revealed some differences in the composition of the population of lymphocytes and the number of regulatory T cells in patients with HER2+ and triple negative breast cancer (BC). Discuss the relationship between the number of suppressor cells and the degree of therapeutic pathomorphosis of tumor.