Open AccessTHE LIPOSOMAL ARANOSA DOES NOT INDUCE AUTOPHAGY
Author(s) -
D. A. Afanasieva,
М. А. Барышникова,
Yu. A. Khochenkova,
P. V. Golyshko,
Dmitry Khochenkov,
Е В Степанова,
Oxana Ryabaya
Publication year - 2015
Publication title -
rossijskij bioterapevtičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 1726-9792
pISSN - 1726-9784
DOI - 10.17650/1726-9784-2015-14-1-15-18
Subject(s) - autophagy , programmed cell death , apoptosis , clone (java method) , cell culture , microbiology and biotechnology , cell , cancer research , chemistry , biology , gene , biochemistry , genetics
Previous studies have shown that aranosa-lio initiate cell death by activation of CD95-receptor of apoptosis. The mechanism of liposomal aranosa action is still unknown. Anticancer drugs may cause tumor cell death by the activation of autophagy. The purpose of this study was to determine the influence of liposomal aranosa on autophagy. The study was assessed on human melanoma cell lines mel Mtp, mel Ibr, mel Kor, mel Z and mel Mtp clone X. The cells were treated with aranosa-lio or liposomal aranosa at concentration 450 μg/ml for 24 hours. The expression of autophagy were analyzed the expression of mRNA Beclin 1. Expression of mRNA Beclin 1 was reduced on the majority of cell lines, which might be due to the fact that the liposomal form of the drug caused cell death by intrinsic apoptosis which is suppose to suppress autophagy.