Open AccessGrape Seed Proanthocyanidin Improves Lipopolysaccharide-Induced Myocardial Toxicity in Mice
Author(s) -
Liu Xianchu,
Sha Li,
Ruyan Chen,
Yue Li,
Huan Peng,
Ming Liu
Publication year - 2021
Publication title -
sains malaysiana
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.251
H-Index - 29
ISSN - 0126-6039
DOI - 10.17576/jsm-2021-5004-06
Subject(s) - lactate dehydrogenase , malondialdehyde , superoxide dismutase , lipopolysaccharide , catalase , tumor necrosis factor alpha , grape seed extract , pharmacology , chemistry , toxicity , creatine kinase , interleukin , oxidative stress , biochemistry , medicine , cytokine , endocrinology , enzyme , pathology , alternative medicine , organic chemistry
Lipopolysaccharide (LPS) is an important pathogenic factor for sepsis which results in cardiovascular diseases and even mortality. Proanthocyanidin, one of the main components in grape seed, has a wide range of biological activities in various diseases. The mouse model was established by intraperitoneal injection with LPS. Grape seed proanthocyanidin (GSP) was administered continuously for 8 days. Our results showed that pre-treatment of GSP dramatically ameliorated the level of creatine kinase (CK) and lactate dehydrogenase (LDH) to defend against LPS-induced myocardial toxicity (p<0.01). Moreover, pre-treatment of GSP significantly mitigated the expression of matrix metallopeptidase 2 (MMP-2) and matrix metallopeptidase 9 (MMP-9) to inhibit LPS-induced myocardial fibrosis (p<0.01). In addition, pre-treatment of GSP significantly increased superoxide dismutase (SOD) and catalase (CAT) activities to improve the level of malondialdehyde (MDA) and hydrogen peroxide (H2O2) (p<0.01). Lastly, pre-treatment of GSP relieved tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) activities to prevent inflammatory responses (p<0.01).