Open AccessPotential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells
Author(s) -
Pablo Eduardo Ocampo,
Viviana Vallejo,
Luis Montoya,
Noeme Sousa Rocha,
Fernanda da Cruz Landim,
S. C. Rahal
Publication year - 2020
Publication title -
revista colombiana de ciencias pecuarias/revista colombiana de ciencias pecuarias
Language(s) - English
Resource type - Journals
eISSN - 2256-2958
pISSN - 0120-0690
DOI - 10.17533/udea.rccp.v34n3a06
Subject(s) - chondrogenesis , mesenchymal stem cell , hyaluronic acid , glycosaminoglycan , extracellular matrix , cartilage , chemistry , osteoarthritis , type ii collagen , stem cell , cellular differentiation , histology , microbiology and biotechnology , pathology , andrology , anatomy , biology , medicine , biochemistry , alternative medicine , gene
Background: Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM.