Open AccessEffect of dehydroepiandroesterone and triamcinolone acetonide on 3T3-L1 cell line
Author(s) -
AUTHOR_ID,
Gisela Gutiérrez-Iglesias
Publication year - 2021
Language(s) - English
Resource type - Dissertations/theses
DOI - 10.17488/rmib.381.25
Subject(s) - keloid , triamcinolone acetonide , fibroblast , dehydroepiandrosterone , cell culture , programmed cell death , cell growth , chemistry , cell , pharmacology , cancer research , medicine , hormone , endocrinology , biology , apoptosis , pathology , biochemistry , immunology , androgen , genetics
The skin in the human is the largest organ, his integrity represents protection against various chemical, biological and mechanical agents. The injuries in this tissue are solved by forming a scar, however, different molecular alterations may overstimulate this process, leading to the formation of aberrant scars (hypertrophic or keloid). The most recommended treatment for such injuries is the intralesional application of triamcinolone acetonide (TA) and on the other hand, dehydroepiandrosterone (DHEA) is a pro-hormone that has a wide variety of biological effects such as regulation of the synthesis of collagen fibers, cell protection, anti-tumor properties, anti-inflammatory and anti-oxidant. In this paper, the combination of AT-DHEA on proliferation and cell death in fibroblast cell line 3T3-L1 was studied. The results showed that the AT 100 and 1000 M DHEA to inhibit proliferation by 50 and 40% respectively. The combination of AT-DHEA (10000-10 M) inhibits cell proliferation and induce programmed cell death, so this combination could be used in hypertrophic or keloid scars for disposal.