Open AccessModel Electrochemical Biosensor for the Detection of Methanol in Aqueous Solutions with Yeast Cells
Author(s) -
Zala Štukovnik,
Urban Bren,
Martin Rozman
Publication year - 2021
Publication title -
acta chimica slovenica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.289
H-Index - 46
eISSN - 1580-3155
pISSN - 1318-0207
DOI - 10.17344/acsi.2020.6545
Subject(s) - biosensor , potentiostat , dielectric spectroscopy , materials science , electrode , yeast , analytical chemistry (journal) , electrochemistry , saccharomyces cerevisiae , chemistry , nanotechnology , chemical engineering , chromatography , biochemistry , engineering
An electrochemical device that serves as a model biosensor and contains yeast Saccharomyces cerevisiae as the active biological element was developed. Different configurations of the electrochemical cells were assembled and tested. Stainless steel was used in the electrochemical cell composition process and the surface of this metal electrode was modified with a thin layer of WO3 if necessary. The yeast Saccharomyces cerevisiae was adhered to the working electrode. The resulting model biosensor was then used to monitor the response to a 10% CH3OH. For detection of biological activity, the electrochemical impedance spectroscopy (EIS) method was applied with a portable potentiostat/galvanostat, where the Bode and the Nyquist plots were interpreted. The stability of the device was beforehand determined by measuring the open circuit potential (OCP). The topography of the electrodes was inspected using the techniques of scanning electron microscopy and optical microscopy. The investigated model biosensor as a case study for the development of more complex biosensors that utilize living cells as the active layer.