Open AccessDifferential Pulse Anodic Voltammetric Determination of Chlorzoxazone in Pharmaceutical Formulation using Carbon Paste Electrode
Author(s) -
S. I. M. Zayed,
Y. M. Issa
Publication year - 2020
Publication title -
acta chimica slovenica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.289
H-Index - 46
eISSN - 1580-3155
pISSN - 1318-0207
DOI - 10.17344/acsi.2020.5731
Subject(s) - chlorzoxazone , horizontal scan rate , chemistry , supporting electrolyte , differential pulse voltammetry , pharmaceutical formulation , carbon paste electrode , buffer solution , electrode , electrochemistry , electrolyte , voltammetry , detection limit , chronoamperometry , analytical chemistry (journal) , chromatography , cyclic voltammetry , organic chemistry , cytochrome p450 , cyp2e1 , enzyme
The electrochemical behavior of chlorzoxazone at the carbon paste electrode was investigated in 0.04 mol/L Britton-Robinson buffer pH 6.50 using cyclic and differential pulse voltammetric techniques. Cyclic voltammetric studies indicated that the oxidation of the drug was irreversible and controlled mainly by diffusion. Experimental and instrumental parameters were optimized (50 mV/s scan rate, 50 mV pulse amplitude, and 0.04 mol/L Britton-Robinson (BR) buffer pH 6.50 as a supporting electrolyte) and a sensitive differential pulse anodic voltammetric method has been developed for the determination of the drug over the concentration range 0.17–1.68 μg/mL chlorzoxazone, with detection and quantitation limits of 0.05 and 0.16 μg/mL, respectively. The proposed voltammetric method was successfully applied to the determinationof the drug in its pharmaceutical formulation (Myoflex tablets), and in spiked human urine samples.