Open AccessEl uso de los RAPDs como marcadores moleculares en plantas
Author(s) -
Adriana Otero Arnaiz,
Marlene de la Cruz,
Ken Oyama
Publication year - 1997
Publication title -
botanical sciences/botanical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.289
H-Index - 17
eISSN - 2007-4476
pISSN - 2007-4298
DOI - 10.17129/botsci.1523
Subject(s) - biology , humanities , microbiology and biotechnology , philosophy
In this paper a review of RAPDs (Random Amplified Polymorphic DNA) as molecular markers is presented. This molecular marker has been used to genetic mapping, population genetic structure, genetic identification, intra and interspecific variation, reconstructing phylogenies and conservation biology. In general, RAPDs have been useful to estimate genetic diversity, genetic identification of taxa, clones, varieties and hybrids. However, their use in Systematic has been questioned due to the difficulties in the determination of identity, homologies and independence of characters. Methods of analyses used to estimate genetic diversity are briefly described. Finally, some recent advances and new technics based on PCR are presented.