Open AccessIsolation and Characterization of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp. lactis BGBM50 Strain
Author(s) -
Nemanja Mirković,
Zorica Radulović,
Gordana Uzelac,
Jelena Lozo,
D. Obradović,
Ljubiša Topisirović,
Milan Kojić
Publication year - 2015
Publication title -
food technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 67
eISSN - 1334-2606
pISSN - 1330-9862
DOI - 10.17113/ftb.53.02.15.3846
Subject(s) - lactococcus lactis , bacteriocin , plasmid , bacteria , strain (injury) , microbiology and biotechnology , gene , lactic acid , biology , chemistry , biochemistry , genetics , anatomy
Lactococcus lactis ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Žanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.