Open AccessRECOVERY OF TOBACCO PLANTS AFTER COPPER IONS APPLICATION
Author(s) -
Anastasia Tugbaeva,
Alexander Ermoshin,
D. S. Plotnikov,
Irina Kiseleva
Publication year - 2020
Publication title -
vestnik permskogo universiteta. seriâ biologiâ
Language(s) - English
Resource type - Journals
ISSN - 1994-9952
DOI - 10.17072/1994-9952-2020-4-344-351
Subject(s) - hydrogen peroxide , lipid peroxidation , superoxide dismutase , chemistry , peroxidase , antioxidant , nicotiana tabacum , superoxide , guaiacol , copper , shoot , dismutase , enzyme , horticulture , biochemistry , botany , biology , organic chemistry , gene
The aftereffects of copper ions (100 and 300 μM) on the organ size, the content of stress markers (hydro-gen peroxide, lipid peroxidation - LPO) and components of the antioxidant status (superoxide dismutase - SOD and guaiacol peroxidase - GPO) were studied in the roots, stem, and leaves of Nicotiana tabacum L. It was shown that tobacco plants were sensitive to long-term exposure to 100 and 300 μM Cu2+. Both concentrations inhibited root growth, while shoot length and thickness, and leaf area decreased only in the case of pretreatment with 300 μM Cu2+. So, the growth of aboveground organs was suppressed signifi-cantly less than the root. The level of lipid peroxidation and the content of hydrogen peroxide in the roots under these conditions were also higher, which indicates a stronger stress, compared to stem and leaf. The activity of antioxidant enzymes SOD and GPO in roots and aboveground organs specifically changed de-pending on the concentration of copper ions and the type of organ. In the roots, there was a decrease in SOD activity and an increase in the activity of GPO under pretreatment with 300 μM Cu2+, compared to the control and 100 μM Cu2+, while in stem and leaves the activity of enzymes decreased. The data ob-tained indicate incomplete plant recovery under the aftereffect of copper ions.