Open AccessCHARACTERISTICS OF BACTERIA ISOLATED FROM THE MINER OF THE VERKHNEKAMSKY SALT DEPOSIT (PERM KRAI)
Author(s) -
A. A. Pyankova,
D. I. Usanina,
Vladislav S. Aleev,
С. М. Блинов,
Е. Г. Плотникова
Publication year - 2020
Publication title -
vestnik permskogo universiteta. seriâ biologiâ
Language(s) - English
Resource type - Journals
ISSN - 1994-9952
DOI - 10.17072/1994-9952-2020-4-312-320
Subject(s) - halophile , halotolerance , 16s ribosomal rna , brine , biology , bacteria , extremophile , microbiology and biotechnology , gammaproteobacteria , halomonas , bacillaceae , bacillus (shape) , bacillales , thermophile , chemistry , genetics , organic chemistry , bacillus subtilis
29 strains of halophilic/halotolerant bacteria were isolated from clay deposits of brine-diverting workings and brine pits of the mine of the Verkhnekamsky salt deposit (Perm krai). As a result of phylogenetic analysis based on a comparison of the 16S rRNA gene sequences, it was found that the isolated cultures are representatives of the classes Gammaproteobacteria (Halomonadaceae and Salinisphaeraceae fami-lies) and Bacilli (family Bacillaceae). Three halophilic strains SHV2, RV14, and SWV1 were similar to the closest type strain of the Salinisphaera hydrothermalis species at the level of 95.94-96.62% (16S rRNA gene), which indicates that these strains belong to a new taxon. Most of the isolated bacteria of the families Halomonadaceae and Bacillaceae are extremophiles: they grow at pH 9-10 and high sa-linity (up to 250-270 g/L NaCl). Two halophilic strains of the genus Halomonas have been found to de-grade salicylic and benzoic acids (decomposition products of polyaromatic compounds), which makes them promising for use in biotechnologies for the restoration of contaminated areas with a high level of mineralization.