Open AccessMICROPROPAGATION OF SOME SORT GROUPS OF LILIES
Author(s) -
Н. Л. Шибанова,
A. S. Popkova
Publication year - 2020
Publication title -
vestnik permskogo universiteta. seriâ biologiâ
Language(s) - English
Resource type - Journals
ISSN - 1994-9952
DOI - 10.17072/1994-9952-2020-4-280-285
Subject(s) - hybrid , lilium , explant culture , micropropagation , biology , cultivar , botany , ornamental plant , horticulture , auxin , sterilization (economics) , in vitro , genetics , gene , economics , monetary economics , foreign exchange market , foreign exchange
The article presents data on micropropagation of ten cultivars of the genus Lilium L. belonging to four groups: the Oriental hybrids, the Trumpet hybrids, Lilium Asiatic hybrids, the Longiflorum and Asiatic hybrids (LA-hybrids). It is found that for the sterilization of explants better use the 7% sodium hypo-chlorite solution (20 min) and 70% ethanol (10 sec). The average percentage of sterile culture for the Ori-ental hybrids was 66.00±7.07, the Trumpet hybrids – 81.00±5.65, Lilium Asiatic hybrids – 85.00±4.69, LA-hybrids – 87.00±6.36. There were no statistically significant differences between cultivars within the group (p=0.33, p<0.05). It is recommended to use a medium Murashige and Skuga with the addition of auxin α-naphthylacetic acid 1 ml/l – for the Oriental, Trumpet, Asiatic hybrids or cytokinin 6-benzylaminopurine 1 m/l – for LA hybrids. The percentage of explant with signs of regeneration was more than 82% for Trumpet and LA hybrids and more than 71% for Oriental and Asiatic hybrids. The reproduction rate can vary from 2 to 14 pcs/explant. Plant survival was more than 60%.