Open AccessMICROPROPAGATION OF GLADIOLUS × HYBRIDUS HORT. ‘ПЕРМСКИЙ СУВЕНИР ’, WHICH WAS BRED IN BOTANICAL GARDEN OF PSU
Author(s) -
Н. Л. Шибанова,
М А Черткова,
Татьяна Дмитриевна Чемарова
Publication year - 2020
Publication title -
vestnik permskogo universiteta. seriâ biologiâ
Language(s) - English
Resource type - Journals
ISSN - 1994-9952
DOI - 10.17072/1994-9952-2020-2-97-102
Subject(s) - corm , micropropagation , gladiolus , horticulture , murashige and skoog medium , biology , sterilization (economics) , kinetin , sodium hypochlorite , botany , explant culture , chemistry , in vitro , foreign exchange market , foreign exchange , biochemistry , organic chemistry , economics , monetary economics
The article presents data on micropropagation of new Gladiolus × hybridus cultivar ‘Пермский Сувенир’. It is found that for the sterilization of corms and cormels better use the 7% sodium hypochlorite solution (15 min or 20-25 min) and then 96% ethanol (30 sec or 60 sec). Sterile culture was more than 56% for corms and 76% for cormels. At the micropropagation good results were obtained when vitamins (0.1 mg/l thiamine, 0.5 mg/l pyridoxine, 0.5 mg/l nicotinic acid) and β-indolylacetic acid (0.1-1 mg/l) were added to the Murashige and Skoog medium. The breeding rate for cormels was 2-3, for corm fragments was 2–4. The survival rate of plants transferred from in vitro to in vivo conditions was 70%. The diameter of the obtained juvenile corms from in vitro culture and the number of cormels formed on them did not have statistically significant differences from those planted in vivo (t = [1.16; 1.22] <t05 = 1.96).