Open AccessIMMOBILIZATION OF CELLS OF NITRILE-HYDROLYZING BACTERIA RHODOCOCCUS ERYTHROPOLIS 4-1 AND ALCALIGENES FAECALIS 2 USING THERMOTROPIC AND IONOTROPIC GELS
Author(s) -
E. M. Mochalova,
Yu. G. Maksimova
Publication year - 2020
Publication title -
vestnik permskogo universiteta. seriâ biologiâ
Language(s) - English
Resource type - Journals
ISSN - 1994-9952
DOI - 10.17072/1994-9952-2020-1-26-32
Subject(s) - alcaligenes faecalis , chemistry , alcaligenes , bacteria , pseudomonas stutzeri , rhodococcus , amidase , chromatography , nuclear chemistry , organic chemistry , hydrolysis , pseudomonas , biology , genetics , enzyme
Cells of amidase containing bacteria of Rhodococcus erythropolis 4-1 and Alcaligenes faecalis 2 were immobilized using carriers such as barium alginate, agarose, chitosan and κ-carrageenan. The aim of the work was to study the effect of bacterial cell immobilization methods on the operational stability of a bio-catalyst. It has been determined that the best carrier for the stable operation of the biocatalyst was aga-rose. The highest amount of acrylic acid was obtained during multi-cycle transformation of acrylamide by immobilized A. faecalis 2 cells.