Open AccessClonal Analysis of Individual Marrow‐Repopulating Cells after Experimental Peripheral Blood Progenitor Cell Transplantation
Author(s) -
Nagy K. Zsuzsanna,
Laufs Stephanie,
Gentner Bernhard,
Naundorf Sonja,
Kuehlcke Klaus,
Topaly Julian,
Buss Eike C.,
Zeller W. Jens,
Fruehauf Stefan
Publication year - 2004
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.22-4-570
Subject(s) - biology , progenitor cell , haematopoiesis , provirus , stem cell , transplantation , bone marrow , immunology , cancer research , genetic enhancement , virology , microbiology and biotechnology , gene , genetics , medicine , genome , surgery
Methods to analyze the clonality of an adverse event in preclinical or clinical retroviral stem cell gene therapy protocols are needed. We analyzed the progeny of retrovirally transduced human peripheral blood progenitor cells (PBPCs) after transplantation and engraftment in immune‐deficient mice. The integration site of the provirus serves as a unique tag of the individual transduced PBPC. A plasmid library of junctions between proviral and human genomic DNA was generated. We were able to detect individual transduced cell clones that amounted to 0.14%–0.0001% of chimeric bone marrow cells. This is the first report in which the contribution of individual marrow‐repopulating cells to human hematopoiesis is directly quantified.