Open AccessBreaking the Species Barrier: Derivation of Germline‐Competent Embryonic Stem Cells from Mus spretus × C57BL/6 Hybrids
Author(s) -
Hochepied Tino,
Schoonjans Luc,
Staelens Jan,
Kreemers Veerle,
Danloy Sophie,
Puimège Leen,
Collen Désiré,
Van Roy Frans,
Libert Claude
Publication year - 2004
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.22-4-441
Subject(s) - biology , embryonic stem cell , genetics , inbred strain , offspring , laboratory mouse , house mouse , chimera (genetics) , germline , phenotype , cloning (programming) , genotype , stem cell , mutagenesis , hybrid , microbiology and biotechnology , mutation , gene , pregnancy , botany , computer science , programming language
Embryonic stem (ES) cells, which can differentiate into almost all types of cells, have been derived from the house mouse Mus musculus , rat, rabbit, humans, and other species. Transmission of the genotype to the offspring of chimeras has been achieved only with M. musculus ES cells, limiting targeted mutagenesis using ES cells to this species. Mus spretus , which exhibits many genetic polymorphisms with M. musculus , displays dominant resistance to cancer and inflammation, making derived inbred strains very useful in positional cloning and interspecies mapping. We show here for the first time the derivation of ES cells from hybrid blastocysts, obtained by the mating of two different species, namely Mus musculus and Mus spretus , and their use for the generation of chimeric mice that transmit the Mus spretus genotype and phenotype to the offspring. These hybrid ES cells allow the genetic manipulation of Mus spretus , as an alternative to Mus musculus.