Open AccessCharacterization of Multipotential Mesenchymal Progenitor Cells Derived from Human Trabecular Bone
Author(s) -
Tuli Richard,
Tuli Suraj,
Nandi Sumon,
Wang Mark L.,
Alexander Peter G.,
HaleemSmith Hana,
Hozack William J.,
Manner Paul A.,
Danielson Keith G.,
Tuan Rocky S.
Publication year - 2003
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.21-6-681
Subject(s) - biology , mesenchymal stem cell , progenitor cell , microbiology and biotechnology , cd34 , population , stem cell , bone marrow , collagenase , immunology , medicine , biochemistry , environmental health , enzyme
The in vitro culture of human trabecular bone‐derived cells has served as a useful system for the investigation of the biology of osteoblasts. The recent discovery in our laboratory of the multilineage mesenchymal differentiation potential of cells derived from collagenase‐treated human trabecular bone fragments has prompted further interest in view of the potential application of mesenchymal progenitor cells (MPCs) in the repair and regeneration of tissue damaged by disease or trauma. Similar to human MPCs derived from bone marrow, a clearer understanding of the variability associated with obtaining these bone‐derived cells is required in order to optimize the design and execution of applicable studies. In this study, we have identified the presence of a CD73 + , STRO‐1 + , CD105 + , CD34 − , CD45 − , CD144 − cell population resident within collagenase‐treated, culture‐processed bone fragments, which upon migration established a homogeneous population of MPCs. Additionally, we have introduced a system of culturing these MPCs that best supports and maintains their optimal differentiation potential during long‐term culture expansion. When cultured as described, the trabecular bone‐derived cells display stem cell‐like capabilities, characterized by a stable undifferentiated phenotype as well as the ability to proliferate extensively while retaining the potential to differentiate along the osteoblastic, adipocytic, and chondrocytic lineages, even when maintained in long‐term in vitro culture.