Open AccessMitochondrial DNA Sequence Heterogeneity of Single CD34 + Cells After Nonmyeloablative Allogeneic Stem Cell Transplantation
Author(s) -
Yao YongGang,
Childs Richard W.,
Kajigaya Sachiko,
McCoy J. Philip,
Young Neal S.
Publication year - 2007
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2007-0269
Subject(s) - biology , stem cell , cd34 , transplantation , mitochondrial dna , haematopoiesis , progenitor cell , microbiology and biotechnology , hematopoietic stem cell , somatic cell , immunology , hematopoietic stem cell transplantation , genetics , gene , medicine
We applied a single‐cell method to detect mitochondrial DNA (mtDNA) mutations to evaluate the reconstitution of hematopoietic stem cells (HSCs) and committed progenitor cells after nonmyeloablative allogeneic stem cell transplantation in humans. In a total of 1,958 single CD34 + cells from six human leukocyte antigen‐matched sibling donor and recipient pairs, individual CD34 + clones were recognized based on the observed donor‐ or recipient‐specific mtDNA sequence somatic alteration. There was no overall reduction of mtDNA heterogeneity among CD34 + cells from the recipient after transplantation. Samples collected from two donors over time showed the persistence of certain CD34 + clones marked by specific mutations. Our results demonstrate the feasibility of distinguishing donor and recipient individual CD34 + clones based on mtDNA mutations during engraftment. HSCs were not limited in number, and similar mtDNA heterogeneity levels suggested representation of the total stem cell compartment during rapid hematopoietic reconstitution in the recipient. Disclosure of potential conflicts of interest is found at the end of this article.