Open AccessAlteration of Marrow Cell Gene Expression, Protein Production, and Engraftment into Lung by Lung‐Derived Microvesicles: A Novel Mechanism for Phenotype Modulation
Author(s) -
Aliotta Jason M.,
SanchezGuijo Fermin M.,
Dooner Gerri J.,
Johnson Kevin W.,
Dooner Mark S.,
Greer Kenneth A.,
Greer Deborah,
Pimentel Jeffrey,
Kolankiewicz Luiz M.,
Puente Napoleon,
Faradyan Sam,
Ferland Paulette,
Bearer Elaine L.,
Passero Michael A.,
Adedi Mehrdad,
Colvin Gerald A.,
Quesenberry Peter J.
Publication year - 2007
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2007-0128
Subject(s) - biology , bone marrow , lung , microvesicles , cell , phenotype , progenitor cell , cancer research , microbiology and biotechnology , immunology , stem cell , pathology , microrna , gene , medicine , genetics , biochemistry
Numerous animal studies have demonstrated that adult marrow‐derived cells can contribute to the cellular component of the lung. Lung injury is a major variable in this process; however, the mechanism remains unknown. We hypothesize that injured lung is capable of inducing epigenetic modifications of marrow cells, influencing them to assume phenotypic characteristics of lung cells. We report that under certain conditions, radiation‐injured lung induced expression of pulmonary epithelial cell‐specific genes and prosurfactant B protein in cocultured whole bone marrow cells separated by a cell‐impermeable membrane. Lung‐conditioned media had a similar effect on cocultured whole bone marrow cells and was found to contain pulmonary epithelial cell‐specific RNA‐filled microvesicles that entered whole bone marrow cells in culture. Also, whole bone marrow cells cocultured with lung had a greater propensity to produce type II pneumocytes after transplantation into irradiated mice. These findings demonstrate alterations of marrow cell phenotype by lung‐derived microvesicles and suggest a novel mechanism for marrow cell‐directed repair of injured tissue. Disclosure of potential conflicts of interest is found at the end of this article.