Open AccessCD41 + /CD45 + Cells Without Acetylcholinesterase Activity Are Immature and a Major Megakaryocytic Population in Murine Bone Marrow
Author(s) -
MatsumuraTakeda Kuniko,
Sogo Shinji,
Isakari Yoshimasa,
Harada Yasuo,
Nishioka Kinue,
Kawakami Takuma,
Ono Toshihide,
Taki Takao
Publication year - 2007
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2006-0363
Subject(s) - acetylcholinesterase , aché , population , megakaryocyte , microbiology and biotechnology , bone marrow , biology , progenitor cell , chemistry , immunology , biochemistry , enzyme , stem cell , medicine , environmental health
Murine megakaryocytes (MKs) are defined by CD41/CD61 expression and acetylcholinesterase (AChE) activity; however, their stages of differentiation in bone marrow (BM) have not been fully elucidated. In murine lineage‐negative (Lin − )/CD45 + BM cells, we found CD41 + MKs without AChE activity (AChE − ) except for CD41 ++ MKs with AChE activity (AChE + ), in which CD61 expression was similar to their CD41 level. Lin − /CD41 + /CD45 + /AChE − MKs could differentiate into AChE + , with an accompanying increase in CD41/CD61 during in vitro culture. Both proplatelet formation (PPF) and platelet (PLT) production for Lin − /CD41 + /CD45 + /AChE − MKs were observed later than for Lin − /CD41 ++ /CD45 + /AChE + MKs, whereas MK progenitors were scarcely detected in both subpopulations. GeneChip and semiquantitative polymerase chain reaction analyses revealed that the Lin − /CD41 + /CD45 + /AChE − MKs are assigned at the stage between the progenitor and PPF preparation phases in respect to the many MK/PLT‐specific gene expressions, including β1‐tubulin. In normal mice, the number of Lin − /CD41 + /CD45 + /AChE − MKs was 100 times higher than that of AChE + MKs in BM. When MK destruction and consequent thrombocytopenia were caused by an antitumor agent, mitomycin‐C, Lin − /CD41 + /CD45 + /AChE − MKs led to an increase in AChE + MKs and subsequent PLT recovery with interleukin‐11 administration. It was concluded that MKs in murine BM at least in part consist of immature Lin − /CD41 + /CD45 + /AChE − MKs and more differentiated Lin − /CD41 ++ /CD45 + /AChE + MKs. Immature Lin − /CD41 + /CD45 + /AChE − MKs are a major MK population compared with AChE + MKs in BM and play an important role in rapid PLT recovery in vivo. Disclosure of potential conflicts of interest is found at the end of this article.