Open AccessEmbryonic Stem Cell‐Derived Neural Progenitors Display Temporal Restriction to Neural Patterning
Author(s) -
Bouhon Isabelle A.,
Joannides Alexis,
Kato Hidemasa,
Chandran Siddharthan,
Allen Nicholas D.
Publication year - 2006
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2006-0031
Subject(s) - biology , gliogenesis , neurogenesis , olig2 , embryonic stem cell , neural stem cell , progenitor cell , stem cell , forebrain , neurosphere , neuroscience , neuroepithelial cell , microbiology and biotechnology , neural cell , adult stem cell , cell , genetics , oligodendrocyte , central nervous system , myelin , gene
Neural stem cells have considerable therapeutic potential because of their ability to generate defined neuronal cell types for use in drug screening studies or cell‐based therapies for neurodegenerative diseases. In this study, we differentiate mouse embryonic stem cells to neural progenitors with an initial forebrain identity in a defined system that enables systematic manipulation to generate more caudal fates, including motoneurons. We demonstrate that the ability to pattern embryonic stem cell‐derived neural progenitors is temporally restricted and show that the loss of responsiveness to morphogenetic cues correlates with constitutive expression of the basic helix‐loop‐helix transcription factors Olig2 and Mash1, epidermal growth factor receptor, and vimentin and parallels the onset of gliogenesis. We provide evidence for two temporal classes of embryonic stem cell‐derived putative radial glia that coincide with a transition from neurogenesis to gliogenesis and a concomitant loss of regional identity.