Open AccessMore Than 40,000 Transcripts, Including Novel and Noncoding Transcripts, in Mouse Embryonic Stem Cells
Author(s) -
Araki Ryoko,
Fukumura Ryutaro,
Sasaki Naokazu,
Kasama Yasuji,
Suzuki Nobuko,
Takahashi Hirokazu,
Tabata Yoshimichi,
Saito Toshiyuki,
Abe Masumi
Publication year - 2006
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2006-0005
Subject(s) - biology , embryonic stem cell , transcriptome , stem cell , gene , gene expression profiling , gene expression , cloning (programming) , microbiology and biotechnology , genetics , computer science , programming language
To study the transcriptome of embryonic stem cells, we used a new gene expression profiling method that can measure the expression levels of unknown and rarely expressed transcripts precisely. We detected a total of 33,136 signal peaks representing transcripts in mouse embryonic stem cells, E14. Subsequent random cloning of the peaks suggests that mouse embryonic stem cells express at least 40,000 transcripts, of which about 2,000 are still unknown. In addition, we identified 1,022 noncoding transcripts, several of which change depending on differentiation in gene expression. Our database provides a high‐resolution expression profile of E14 cells and is applicable to other mouse embryonic stem cell analyses. It includes most transcription regulation factor‐encoding genes and a significant number of unknown and noncoding transcripts.