Open AccessGranulocyte Colony‐Stimulating Factor Mobilizes More Dendritic Cell Subsets Than Granulocyte‐Macrophage Colony‐Stimulating Factor with No Polarization of Dendritic Cell Subsets in Normal Donors
Author(s) -
Shaughnessy Paul J.,
Bachier Carlos,
LeMaistre Charles F.,
Akay Cagla,
Pollock Brad H.,
Gazitt Yair
Publication year - 2006
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2005-0492
Subject(s) - biology , granulocyte macrophage colony stimulating factor , dendritic cell , granulocyte colony stimulating factor , immunology , granulocyte , haematopoiesis , antigen presenting cell , cytokine , microbiology and biotechnology , stem cell , antigen , t cell , immune system , genetics , chemotherapy
Dendritic cells (DCs) are effective antigen‐presenting cells. We hypothesized that increasing the DC populations in donor lymphocyte infusions (DLIs) may augment the graft versus malignancy effect, particularly if granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) mobilization resulted in increased precursor dendritic cell (pDC) 1 cells. Mature DCs, pDC1 cells, pDC2 cells, and CD34 + cells from the same donor were compared after granulocyte colony‐stimulating factor (G‐CSF) mobilized peripheral blood stem cell collections and GM‐CSF mobilized DLI collections. Mobilization with G‐CSF resulted in up to a 10‐fold larger number of CD34 + cells per kg and a 3–5‐fold larger number of mature DCs, pDC1 cells, and pDC2 cells within the same donor compared with GM‐CSF. The ratio of pDC1 to pDC2 in each donor remained constant with either cytokine. In this small sample of normal donors, it appears that G‐CSF mobilizes more CD34 + cells, mature DCs, pDC1 cells, and pDC2 cells within the same donor than does GM‐CSF, with no significant polarization by G‐CSF or GM‐CSF for either pDC1 or pDC2 cells.