Open AccessMechanisms Underlying Acceleration of Blood Flow Recovery in Ischemic Limbs by Macrophage Colony‐Stimulating Factor
Author(s) -
Nakano Keiji,
Adachi Yasushi,
Minamino Keizo,
Iwasaki Masayoshi,
Shigematsu Akio,
Kiriyama Naoko,
Suzuki Yasuhiro,
Koike Yasushi,
Mukaide Hiromi,
Taniuchi Shoichiro,
Kobayashi Yohnosuke,
Kaneko Kazunari,
Ikehara Susumu
Publication year - 2006
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2005-0421
Subject(s) - bone marrow , vascular endothelial growth factor , progenitor cell , neovascularization , biology , macrophage colony stimulating factor , myeloid , macrophage , ischemia , angiogenesis , mobilization , immunology , stem cell , medicine , cancer research , microbiology and biotechnology , vegf receptors , in vitro , history , biochemistry , archaeology
Recently we reported that macrophage colony‐stimulating factor (M‐CSF) can mobilize endothelial progenitor cells (EPCs) from the bone marrow into the peripheral blood, resulting in an increase in the number of blood vessels and augmentation of blood flow in the ischemia‐induced legs. M‐CSF accelerates neovascularization of ischemic lesions resulting from the mobilization of EPCs. In the present paper, we analyze the mechanisms underling the mobilization of EPCs by M‐CSF. M‐CSF augments the production of vascular endothelial growth factor (VEGF) from the bone marrow cells, especially from myeloid lineage cells. In vivo administration of anti‐VEGF antibody abrogates both the acceleration of the recovery of blood flow in the ischemia‐induced limbs by M‐CSF and the augmentation of the mobilization of EPCs induced by M‐CSF. These results suggest that the M‐CSF contributes to rapid recovery of blood flow in ischemic lesions by mobilization of EPCs from the bone marrow through augmentation of VEGF production in the bone marrow and that the VEGF is mainly produced by myeloid lineage cells.