Open AccessCharacterization of Mesenchymal Stem Cells Isolated from Mouse Fetal Bone Marrow
Author(s) -
Wang Xiaoli,
Hisha Hiroko,
Taketani Shigeru,
Adachi Yasushi,
Li Qiang,
Cui Wenhao,
Cui Yunze,
Wang Jianfeng,
Song Changye,
Mizokami Tomomi,
Okazaki Satoshi,
Li Qing,
Fan Tianxue,
Fan Hongxue,
Lian Zhexiong,
Gershwin M. Eric,
Ikehara Susumu
Publication year - 2006
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2005-0219
Subject(s) - mesenchymal stem cell , biology , haematopoiesis , bone marrow , stromal cell , stem cell , microbiology and biotechnology , stem cell transplantation for articular cartilage repair , cytokine , endothelial stem cell , immunology , stem cell factor , adult stem cell , in vitro , cancer research , biochemistry
Mesenchymal stem cells (MSCs) are defined as cells that can differentiate into multiple mesenchymal lineage cells. MSCs have some features (surface molecules and cytokine production, etc.) common to so‐called traditional bone marrow (BM) stromal cells, which have the capacity to support hemopoiesis. In the present study, we isolated murine MSCs (mMSCs) from the fetal BM using an anti‐PA6 monoclonal antibody (mAb) that is specific for bone marrow stromal cells. The mMSCs, called FMS/PA6‐P cells, are adherent, fibroblastic, and extensively expanded and have the ability to differentiate not only into osteoblasts and adipocytes but also into vascular endothelial cells. The FMS/PA6‐P cells produce a broad spectrum of cytokines and growth factors closely related to hemopoiesis and show good hemopoiesis‐supporting capacity both in vivo and in vitro, suggesting that they are a component of the hemopoietic stem cell niche in vivo. Interestingly, although the FMS/PA6‐P cells express a high level of the PA6 molecule, which is reactive with anti‐PA6 mAb, they gradually lose their ability to express this molecule during the course of differentiation into osteoblasts and adipocytes, indicating that the PA6 molecule might serve as a novel marker of mMSCs.