Open AccessMyocardial Homing of Nonmobilized Peripheral‐Blood CD34 + Cells After Intracoronary Injection
Author(s) -
Blocklet Didier,
Toungouz Michel,
Berkenboom Guy,
Lambermont Micheline,
Unger Philippe,
Preumont Nicolas,
Stoupel Eric,
Egrise Dominique,
Degaute JeanPaul,
Goldman Michel,
Goldman Serge
Publication year - 2006
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2005-0201
Subject(s) - homing (biology) , cd34 , myocardial infarction , stem cell , haematopoiesis , medicine , cardiology , biology , microbiology and biotechnology , ecology
Granulocyte– colony‐stimulating factor administered for autologous hematopoietic stem cell isolation from blood may favor restenosis in patients implanted after acute myocardial infarction (AMI). We therefore tested the isolation of peripheral‐blood CD34 + cells without mobilization in six patients with AMI. After large‐volume cytapheresis and positive CD34 + cell selection, 3.6 to 27.6 million CD34 + cells were obtained. We performed intra‐coronary implantation of these cells and recorded no restenosis or arrhythmia. We used positron emission tomography (PET) to assess myocardial‐labeled CD34 + cell homing, which accounted for 5.5% of injected cells 1 hour after implantation. In conclusion, large amounts of CD34 + cells, in the range reported in previous studies, can be obtained from nonmobilized peripheral blood. PET with [ 18 F]‐fluorodeoxyglucose cell labeling is an efficient imaging method for homing assessment.