Open AccessIn Vivo and In Vitro Tissue‐Specific Expression of Green Fluorescent Protein Using the Cre‐Lox System in Mouse Embryonic Stem Cells
Author(s) -
Schindehütte Jan,
Fukumitsu Hidefumi,
Collombat Patrick,
Griesel Gundula,
Brink Christopher,
Baier Paul C.,
Capecchi Mario R.,
Mansouri Ahmed
Publication year - 2005
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2004-0163
Subject(s) - biology , embryonic stem cell , stem cell , microbiology and biotechnology , green fluorescent protein , induced pluripotent stem cell , adult stem cell , transgene , transplantation , cellular differentiation , gene targeting , gene , genetics , medicine , surgery
Embryonic stem cells (ES) are pluripotent and may therefore serve as a source for the generation of specific cell types required for future therapies based on cell replacement. The isolation of defined cell populations from a certain lineage or tissue is a prerequisite for the analysis of the potential of such ES‐derived cells in animal transplantation studies. Here, using the Cre/loxP system, we report the generation of murine ES cells conditionally expressing the hrGFP gene at the cell surface. Such ES cells can be differentiated in vitro into neurons displaying GFP activity in neurites. Transgenic mice derived from these ES cells permit the targeting of GFP‐expression to specific tissues and provide material from the three germ layers suitable for molecular and biochemical analysis.