Open AccessMarked Increase in Number of Dendritic Cells in Autoimmune‐Prone (NZW × BXSB)F1 Mice with Age
Author(s) -
Adachi Yashusi,
Taketani Shigeru,
Toki Junko,
Ikebukuro Kazuya,
Sugiura Kikuya,
Oyaizu Haruki,
Yasumizu Ryoji,
Tomita Minoru,
Kaneda Hiroyuki,
Amoh Yasuo,
Ito Tomoki,
Okigaki Mitsuhiko,
Inaba Muneo,
Ikehara Susumu
Publication year - 2002
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.20-1-61
Subject(s) - cd11c , biology , haematopoiesis , cd3 , immunology , bone marrow , microbiology and biotechnology , dendritic cell , medicine , endocrinology , immune system , cd8 , stem cell , biochemistry , gene , phenotype
Here, we report that the number of CD11c + CD3 − B220 − cells increases in autoimmune‐prone male (NZW × BXSB)F1 (W/BF1) mice with age. The CD11c + CD3 − B220 − cells from W/BF1 mice show a typical stellate shape and induce the proliferation of T cells. In the CD11c + CD3 − B220 − cells from W/BF1 mice, CD11b (Mac‐1α), NK 1.1, and CD95 (Fas) are upregulated in comparison with normal mice, while the expression of CD8α, CD117 (c‐kit), CD135 (Flk‐2/Flt‐3), and Sca‐1 decreases. There is a significant increase in Flt‐3L (FL) mRNA in the bone marrow of W/BF1 mice with age. Moreover, activated hemopoietic cells express high levels of FL. The injection of CD11c + CD3 − B220 − cells from old W/BF1 mice to young W/BF1 mice transiently induces autoimmune disease (thrombocytopenia). These results suggest that hyperproduction of FL from activated hemopoietic cells induces a dramatic increase in the number of dendritic cells in aged W/BF1 mice, followed by the acceleration of autoimmunity.