Open AccessBiofilm Formation of Escherichia coli on Hydrophobic Steel Surface Provided by Laser-Texturing : Investigation of modified surfaces to prevent biofilm formation
Author(s) -
Simge Arkan-Ozdemir,
Nurhan Cansever,
Esra IlhanSungur
Publication year - 2023
Publication title -
johnson matthey technology review
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.571
H-Index - 49
ISSN - 2056-5135
DOI - 10.1595/205651322x16457881164562
Subject(s) - biofilm , escherichia coli , scanning electron microscope , lozenge , bacteria , chemistry , microbiology and biotechnology , materials science , extracellular polymeric substance , adhesion , chemical engineering , composite material , biology , biochemistry , history , genetics , archaeology , engineering , gene
Lozenge-patterned (LP) surface obtained with laser texturing can reduce the risk of infection by preventing/delaying the biofilm formation of Escherichia coli. To investigate this aspect, the biofilm formation ability of E. coli on both LP and untreated surfaces of 630 stainless steel was examined over 48 h. Biofilm on the coupons was analyzed for bacterial enumeration and total carbohydrates concentration, and also was observed using scanning electron microscopy. The surface modification by texturing caused a 6 h delay in the attachment of E. coli, and an approximately 99% decrease in the number of adhered bacteria was determined at the end. However, it was determined that E. coli produced more extracellular polymeric substances (EPS) (p<0.01) to attach to the LP surface and formed a multi-layered biofilm. In conclusion, LP surface can be recommended regarding the bacterial count and the delay in attachment, but the increased amount of EPS limits its use.