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Production of Interferon‐γ and Interleukin‐10 After Inactivated Hepatitis A Immunization
Author(s) -
Hayney Mary S.,
Buck Jessica M.,
Muller Daniel
Publication year - 2003
Publication title -
pharmacotherapy: the journal of human pharmacology and drug therapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.227
H-Index - 109
eISSN - 1875-9114
pISSN - 0277-0008
DOI - 10.1592/phco.23.4.431.32127
Subject(s) - immunization , medicine , virology , immunology , interferon , antibody
Study Objective. To assess helper T cell function by measuring cytokine production over time after hepatitis A immunization. Design. Open‐label, single‐dose study. Setting. General clinical research center of a university hospital. Subjects. Twenty‐five healthy adults. Intervention. Each subject was immunized with inactivated hepatitis A vaccine; blood was drawn on day 0 (the day of immunization) and days 2, 5, 7, 10, and 28 after immunization. Measurements and Main Results. Production of interferon (IFN)‐γ and interleukin (IL)‐10 by peripheral blood mononuclear cells stimulated in culture with hepatitis A virus was measured by enzyme‐linked immunosorbent assay. Concentrations of hepatitis A antibody were measured on day 28. Both IFN‐γ and IL‐10 production peaked on day 10 after immunization (IFN‐γ day 0 median = 7.35 pg/ml, interquartile ratio [IQR] = 20.8 vs day 10 median = 22.35 pg/ml, IQR = 42.4, p<0.05; IL‐10 day 0 median = 1.00, IQR = 7.4 vs day 10 median = 11.75 pg/ml, IQR = 92.3, p<0.02, Wilcoxon signed rank test). The IL‐10:IFN‐γ ratio on day 10 correlated with antibody production (Pearson product moment correlation 0.46, p<0.05). This ratio was used as a measure of helper T cell phenotype. Conclusion. Both IFN‐γ and IL‐10 are produced in response to hepatitis A vaccine. The parallel production after immunization may contribute to the high efficacy of these vaccine preparations in inducing both cell‐mediated immune response and a protective antibody response.