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Effects of Adjunctive Treatment with Combined Cytokines in a Neutropenic Mouse Model of Multidrug‐Resistant Enterococcus faecalis Septicemia
Author(s) -
Nicolau David P.,
Onyeji Cyprian O.,
Banevicius Mary Anne,
Li Jing,
Nightingale Charles H.
Publication year - 2001
Publication title -
pharmacotherapy: the journal of human pharmacology and drug therapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.227
H-Index - 109
eISSN - 1875-9114
pISSN - 0277-0008
DOI - 10.1592/phco.21.3.275.34209
Subject(s) - medicine , vancomycin , granulocyte colony stimulating factor , adjunctive treatment , regimen , antibiotics , gentamicin , enterococcus faecalis , neutropenia , pharmacology , gastroenterology , staphylococcus aureus , microbiology and biotechnology , chemotherapy , biology , bacteria , genetics
Study Objective. To examine whether the antienterococcal efficacy of a regimen of gentamicin plus vancomycin combined with granulocyte colony‐stimulating factor (G‐CSF) is enhanced by concurrent therapy with interferon‐γ (IFN‐γ). Setting. Hospital laboratory. Intervention. Mice rendered neutropenic by cyclophosphamide were intraperitoneally inoculated with a gentamicin‐ and vancomycin‐resistant Enterococcus faecalis isolate. Measurements and Main Results. Infected animals were randomized into treatment groups that received G‐CSF alone or in combination with various dosages of IFN‐γ. Additional groups of animals received vancomycin; G‐CSF, G‐CSF plus vancomycin, IFN‐γ, and G‐CSF; or vancomycin with both cytokines. Addition of IFN‐γ to G‐CSF regimen resulted in no significant change (p>0.05) in survival, compared with treatment with G‐CSF alone. Also, the antienterococcal efficacy of antibiotic plus G‐CSF was not modified by coadministration of IFN‐γ. Conclusion. This study suggests that adjunctive application of combined cytokines may not be more beneficial than only G‐CSF in combination with an antibiotic to treat multidrug‐resistant enterococcal infection.