Open AccessEsterase electrophoresis of Clostridium perfringens bovine strains
Author(s) -
Lúcia Baldassi,
Maria Luisa Barbosa,
Erna Elisabeth Bach,
Sebastião Timo Iaria
Publication year - 2003
Publication title -
the journal of venomous animals and toxins including tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.51
H-Index - 30
eISSN - 1678-9199
pISSN - 1678-9180
DOI - 10.1590/s1678-91992003000200009
Subject(s) - clostridium perfringens , typing , esterase , biology , microbiology and biotechnology , electrophoresis , gel electrophoresis , bacteria , genetics , enzyme , biochemistry
Eighty-nine of 144 isolates of Clostridium perfringens obtained from 187 samples of 71 bovine in several Brazilian states were submitted to esterase electrophoresis for typing. Mobilites electrophoresis, as parameter, were settled down by isolates from ATCC pattern of types A, B, C, and D. Of the 89 isolates, 43 (48.3%) were characterized as electrophoretic type A, 20 (22.5%) as D, 18 (20.2%) as C, and 3 (3.4%) as B. Five (5.6%) isolates did not identity with any type. Similarly, esterase electrophoresis enabled the typing of 94.4 % of the isolates, demonstrating to be an appropriate method for animal sample analyses