Open AccessRestoration of adenosine deaminase, histamine, and IgE in organs of mice injected with cobra venom followed by specific treatment and reversal period
Author(s) -
B. V. Lipps
Publication year - 2003
Publication title -
the journal of venomous animals and toxins including tropical diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.51
H-Index - 30
eISSN - 1678-9199
pISSN - 1678-9180
DOI - 10.1590/s1678-91992003000200007
Subject(s) - histamine , venom , immunoglobulin e , adenosine deaminase , cobra , pharmacology , chemistry , adenosine , medicine , immunology , biology , endocrinology , biochemistry , antibody , computer science , programming language
Adenosine deaminase (ADA), histamine, and IgE are endogenously present in animals. Research from this laboratory reported decreased levels of these substances in organs of mice as a consequence of sub-lethal injection of Naja kaouthia venom. This research reports that decreased ADA, histamine, and IgE levels were prevented by specific treatment and prolonged recovery periods. Adult Balb/c mice injected IM with sub-lethal venom dose were divided into five groups. Group 1 were injected with PBS; Group 2 with anti-cobra venom; and Group 3 with lethal toxin neutralizing factor (LTNF). Groups 4 and 5 were treated with IM or oral synthetic LT-10. After 24 hours, mice were sacrificed and organ homogenates were assayed for ADA, histamine, and IgE. Group 1 showed substantial reduction in levels of these substances. It was revealed that decreased levels were prevented by treatment with anti-cobra venom, LTNF, and LT-10. In a second series of experiments, venom-injected mice were sacrificed after 3, 7, and 10 days and organs assayed for ADA, histamine, and IgE levels. The recovery period to homeostasis for ADA, histamine, and IgE was 7 to 10 days