Open AccessUse of lymphocyte cultures for BrdU replication banding patterns in anuran species (Amphibia)
Author(s) -
Sanae Kasahara,
Ana Paula Zampieri Silva,
S. Gruber
Publication year - 1998
Publication title -
genetics and molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.549
H-Index - 55
eISSN - 1678-4685
pISSN - 1415-4757
DOI - 10.1590/s1415-47571998000400011
Subject(s) - biology , bromodeoxyuridine , replication (statistics) , lymphocyte , zoology , virology , genetics , cell growth
We describe the standardization of lymphocyte culture procedures in order to improve cytological preparations of anuran species. This methodology permits the use of 5-bromodeoxyuridine (BrdU) treatment to obtain replication banding patterns in the chromosomes of these species. Descreve-se a padronização da cultura de linfócitos com a finalidade de melhorar as preparações citológicas de espécies de anfíbios anuros. Esta metodologia permite o uso do tratamento pelo BrdU para se obter padrões de bandas de replicação nos cromossomos dessas espécies