Open AccessToxoplasma gondii: II. Caracterização antigênica de taquizoítos de oito amostras
Author(s) -
Regina Mitsuka,
Italmar Teodorico Navarro,
Andrea Cláudia Beckner Da Silva,
José Wander Breganó,
A.A. Alfieri,
José Vítor Jankevicius,
Odilon Vidotto
Publication year - 1998
Publication title -
brazilian journal of veterinary research and animal science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.187
H-Index - 17
eISSN - 1678-4456
pISSN - 1413-9596
DOI - 10.1590/s1413-95961998000300003
Subject(s) - antigen , toxoplasma gondii , heterologous , western blot , microbiology and biotechnology , biology , strain (injury) , virology , antibody , chemistry , immunology , gene , biochemistry , anatomy
Eight Toxoplasma gondii strains were analyzed using ELISA and Western blot techniques, in order to demonstrate possible immunological differences. The analyzed strains were: LIV IV, LIV V and S 11 isolated from swine, RH and VPS from a human being, AS 28 from a wild mouse, HV III from a dog and CN from a cat. With the ELISA assay the eight strains showed similar reactivity with homologous and heterologous sera. The antigenic suspension, consisting of total cellular extract of tachyzoites, was effective in the indirect ELISA assay, with the positive sera reacting strongly and the negative not reacting with the antigens. The Western blot analysis showed that the T. gondii strains have similar antigenic profiles with a few variations. Three bands were observed in all strains: one of about 33 kDa (p33), another of 54 kDa (p54) and a third one of 66 kDa (p66). The HV III strain, isolated from a dog, did not show three antigens (50, 70 and 75 kDa) that were present in the others. However, this difference was not detected by the ELISA assay. Only two antigens (62 kDa of the CN and 67 kDa of the LIV IV) were strain-specific antigens