Open AccessMicrurus spixii (PERUVIAN CORAL SNAKE) VENOM - PRELIMINARY BIOCHEMICAL AND ENZYMATIC CHARACTERIZATION
Author(s) -
César Remuzgo,
Myriam Paola Alvarez,
Edith Rodríguez,
Fanny Lazo,
Armando Yarlequé
Publication year - 2002
Publication title -
the journal of venomous animals and toxins/the journal of venomous animals and toxins
Language(s) - English
Resource type - Journals
eISSN - 1678-4936
pISSN - 0104-7930
DOI - 10.1590/s0104-79302002000100012
Subject(s) - venom , sephadex , ophidia , enzyme , snake venom , size exclusion chromatography , alkaline phosphatase , crotalus , biochemistry , hyaluronidase , chemistry , elapidae , acetylcholinesterase , biology
Micrurus spixii venom was studied after fractionation by Sephadex G-100 SF gel filtration chromatography. Several enzymatic activities and biological effects were investigated in whole venom and fractions. The venom was resolved in four peaks in a range of about 73.2-10.7 kDa molecular weight. Alkaline phosphatase and acetylcholinesterase activities were found in peak I, and procoagulant activity was seen in peak II. Phospholipase A2, hemorrhagic, and proteolytic activities were detected in peak III. A second procoagulant factor and proteinase were present in peak IV. Thrombin-like enzyme and direct hemolytic activities were not found in any assayed samples