Open AccessAFM characterization of protein net formation on a fibrous medium
Author(s) -
O. B. G. Assis,
Denise Cristina Moretti Vieira,
Rubens Bernardes-Filho
Publication year - 2000
Publication title -
brazilian journal of chemical engineering/brazilian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.313
H-Index - 52
eISSN - 1678-4383
pISSN - 0104-6632
DOI - 10.1590/s0104-66322000000200013
Subject(s) - atomic force microscopy , lysozyme , characterization (materials science) , chemical engineering , membrane , materials science , aqueous medium , permeability (electromagnetism) , surface finish , surface roughness , aqueous solution , chemistry , nanotechnology , composite material , biochemistry , organic chemistry , engineering
Lysozyme protein net is set on a glass fiber support using the self-assembly technique. Enzymatic film formation is followed by surface imaging via atomic force microscopy (AFM). Change in roughness as a function of deposition time is used as an indirect indicator of film formation. The objective was to form a protein film that would have no effect on the permeability of the medium, aiming at its application as a bioactive membrane or reactor suitable for bacteria and chemical interactions in aqueous media