Open AccessInfluence of the reaction products in the inversion of sucrose by invertase
Author(s) -
Ubirajara Coutinho Filho,
Carla Eponina Hori,
Eloízio Júlio Ribeiro
Publication year - 1999
Publication title -
brazilian journal of chemical engineering/brazilian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.313
H-Index - 52
eISSN - 1678-4383
pISSN - 0104-6632
DOI - 10.1590/s0104-66321999000200006
Subject(s) - sucrose , invertase , fructose , chemistry , hydrolysis , non competitive inhibition , kinetics , enzyme , biochemistry , chromatography , physics , quantum mechanics
In this work a kinetic model for the enzymatic conversion of sucrose to glucose and fructose using the free form of the enzyme invertase was studied. The initial rates of conversion of sucrose into glucose and fructose were evaluated for different initial concentrations of sucrose, fructose and glucose. The results of the ?-square test showed that the partially non competitive inhibition model for glucose and for fructose can be used to describe the reaction kinetics with a degree of confidence higher than 99.9%. An analysis of the sum of squares of the residuals for several inhibition models cited in the literature showed that the partially noncompetitive inhibition model is the best one for quantifying the effect of the concentration of glucose and fructose on sucrose hydrolysis. A comparison of a simulation of this model with the experimental results showed that the model can describe well the reaction in a batch reactor using initial concentrations of 600 g/L