Open AccessQUANTIFICATION OF THE EFFECT OF SOME OPERATIONAL VARIABLES ON THE CELL GROWTH YIELD (Yx/s) OF Penilcilium chrysogenum BY SURFACE RESPONSE ANALYSIS
Author(s) -
M.E.A.G. Oprime,
Cláudio Alberto Torres Suazo
Publication year - 1997
Publication title -
brazilian journal of chemical engineering/brazilian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.313
H-Index - 52
eISSN - 1678-4383
pISSN - 0104-6632
DOI - 10.1590/s0104-66321997000400017
Subject(s) - ammonium sulfate , penicillium chrysogenum , yield (engineering) , corn steep liquor , chemistry , sucrose , response surface methodology , ammonium , chromatography , food science , materials science , biochemistry , fermentation , organic chemistry , metallurgy
The yield coefficient Yx/s was correlated with variables such as temperature (22.8-29.2 oC), concentrations of corn steep liquor (6.4-57.4 g/L), ammonium sulfate (5.6-18.4 g/L), sucrose (4.0-36.0 g/L) and soybean oil (3.0-7.0 g/L), all important variables in the growth stage of the Penicillium chrysogenum fungus. An empirical model was obtained by response surface statistical experimental design. The tests were performed with a complex culture medium in a rotatory shaker. The highest Yx/s values were obtained at low sucrose concentrations (~4.0 g/L) and high corn steep liquor concentrations (~57.4 g/L). Temperature, ammonium sulfate and soybean oil concentrations showed no significant effect on Yx/