Open AccessA SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION
Author(s) -
Andreas Gombert,
Beatriz Vahan Kilikian
Publication year - 1997
Publication title -
brazilian journal of chemical engineering/brazilian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.313
H-Index - 52
eISSN - 1678-4383
pISSN - 0104-6632
DOI - 10.1590/s0104-66321997000200011
Subject(s) - escherichia coli , plasmid , recombinant dna , symbol (formal) , font , biology , chemistry , microbiology and biotechnology , computer science , biochemistry , gene , artificial intelligence , programming language
- A cultivation strategy based on some previous knowledge of the metabolism of Escherichia coli BL21 (DE3) pLysS containing the troponin C gene cloned into plasmid pET was developed and applied through the use of simple fermentation equipment and a feed-forward control strategy in order to achieve a high cell concentration ¾ 92 g l-1 dry cell weight ¾ and a high cell productivity ¾ 3.7 g l-1 h-1