Open AccessDetection of codon 12 mutation in the k-ras oncogene in pancreatic tumors
Author(s) -
Márcia Saldanha Kubrusly,
Euclides Matheucci,
Kátia Ramos Moreira Leite,
Ana Maria M. Coelho,
Osmar Monte,
Marcel Cerqueira César Machado,
H W Pinotti
Publication year - 1999
Publication title -
revista do hospital das clínicas
Language(s) - Portuguese
Resource type - Journals
eISSN - 1678-9903
pISSN - 0041-8781
DOI - 10.1590/s0041-87811999000100004
Subject(s) - adenocarcinoma , mucinous cystadenoma , pancreatic cancer , oncogene , mutation , pancreas , biology , cancer research , gene , pathology , cancer , medicine , genetics , endocrinology , cell cycle
Mutations at codons 12, 13, or 61 of the H-ras, K-ras, and N-ras have been detected in human neoplasias by a variety of techniques. Some of these techniques are very sensitive and can detect K-ras mutation in 90% of the cases of pancreatic adenocarcinomas. We analyzed 11 samples of pancreatic adenocarcinoma, three samples of pancreatic mucinous cystadenoma, and two samples without tumors in formalin-fixed paraffin embedded tissue sections. K-ras mutations at codon 12 were detected by a two-step PCR-enriched technique in all the samples of pancreatic adenocarcinoma, but not in cystadenoma or control samples. This technique may be useful for early detection of pancreatic cancer.