Open AccessAntibiotic Resistance Pattern of Bacterial Pathogens Isolated from Cow Dung Used to Fertilize Nigerian Fish Ponds
Author(s) -
Funso S. Omojowo,
Patricia Folakemi Omojasola
Publication year - 2013
Publication title -
notulae scientia biologicae
Language(s) - English
Resource type - Journals
eISSN - 2067-3264
pISSN - 2067-3205
DOI - 10.15835/nsb518998
Subject(s) - nalidixic acid , microbiology and biotechnology , biology , ampicillin , gentamicin , tetracycline , aeromonas hydrophila , antibiotic resistance , amoxicillin , ofloxacin , cow dung , chloramphenicol , erythromycin , amikacin , veterinary medicine , antibiotics , ciprofloxacin , bacteria , medicine , ecology , fertilizer , genetics
This study was carried out to isolate and identify antibiotic resistant bacteria from cow dung used for pond fertilization. Cow dung from over 200 cows in NIFFR integrated farms, New-Bussa, Nigeria were collected. Six bacterial pathogens; Escherichia coli, E. coli O157:H7, Shigella dysentariae, Staphylococcus aureus, Salmonella typhi, and Aeromonas hydrophila were isolated. Antibiotic susceptibility testing by the disk diffusion method was conducted using ofloxacin, amoxicillin, tetracycline, ampicillin, erythromycin, gentamicin, nalidixic acid and chloramphenicol. All the isolated organisms were 100% sensitive to ofloxacin. The multiple resistance patterns revealed that 100% were resistant to tetracycline, ampicillin (85.6%), amoxicillin (83.3%), chloramphenicol (66%), gentamicin (47.6%), erythromycin (44.4%) and nalidixic acid (18.3%). The Public Health risks posed by the cow dung manure include proliferation of ponds with these organisms that are pathogenic to fish and man, contamination of the environment and the possible retention of these organisms in the table size fish.